Caution

enzyme will denature below pH 4.0.

General description

Native ascorbate oxidase from Cucurbita species. A multi-copper-containing oxidase that catalyzes oxidation of ascorbic acid to dehydroascorbic acid. AO catalyses the four-electron reduction of dioxygen to water with concominant one-electron oxidation of the reducing organic substrate. Suitable for eliminating interference of ascorbic acid in clinical assays and for determination of ascorbic acid. Inhibited by azide, dithiocarbamate, and thiocyanate.

Native ascorbate oxidase from Cucurbita species. Catalyzes oxidation of ascorbic acid to dehydroascorbic acid. Suitable for eliminating interference of ascorbic acid in clinical assays and for determination of ascorbic acid. Inhibited by azide, dithiocarbamate, and thiocyanate.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Packaging

1000 u in Plastic ampoule

Reconstitution

Following reconstitution refrigerate (4°C). This Stock solutions are stable for up to 3 weeks at 4°C.

Unit Definition

One unit is defined as the amount of enzyme that will oxidize 1.0 µmol ascorbic acid per min at 37°C, pH 5.6.

Warning

Toxicity: Standard Handling (A)